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cose isomerase is used for the production of high fructose syrup the reaction is glucose fructose calculate the equilibrium conv

cose isomerase is used for the production of high fructose syrup the reaction is glucose fructose calculate the equilibrium conv


Glucose isomerase


Glucose isomerase, also known as d-xylose isomerase, is a water-soluble enzyme. In 1957, it was discovered early in pseudomonas hydrophila that (GI) can catalyze the isomerization of d-glucose to d-fructose, especially in the production of fructose syrup, which is the key enzyme for the large-scale preparation of high fructose corn syrup from starch in industry. The enzyme can also isomerize xylan into xylose and then produce ethanol by microbial fermentation. With this enzyme, over 90% of the sugar in the grape syrup can be converted into fruit essence for a long time, which greatly increases the sweetness. Therefore, starch can be used as raw material to produce edible high fructose corn syrup.


2. Glucose isomerase producing microorganisms: many glucose isomerase producing strains mainly include salmonella, escherichia coli, bacillus subtilis, staphylococcus, Streptomyces and other bacteria. Most are isolated from the soil. Actinomycetes have the advantages of high yield of glucose isomerase and good thermal stability of enzyme. And the enzyme reaction does not need to add arsenate or manganese salt and other toxic substances. Xylose is usually used as carbon source to isolate isomerase producing bacteria.


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